The amphioxus (or Lancelet) is a promising animal model in evolutionary understanding of organ development from a view of invertebrate-vertebrate transition. Unfortunately, current amphioxus genomes are not good enough to support reliable -omics research, in particular, help representing high quality transcriptome. Here, we constructed an integrative transcript library for Branchiostoma floridae in basis of heterogeneous RNA-seq datasets, including multiple transcriptomes determined at serial development stages using the next-generation sequencing (NGS) and a full-length cDNA library determined by the third-generation sequencing (TGS). This integrative library was tested to outperform amphioxus genome in reference-based transcriptome assembly, as well as the de novo solution. Therefore, the integrative transcript library can be an alternative and complementary solution to the genome in large scale -omics studies. It will be particularly useful for systematic and dynamic exploration of gene behavior.
About the amphioxus (Branchiostoma floridae) Supplied by Prof. Yiquan Wang, Asso. Prof. Guang Li, Developmental Genetics and Comparative Genomics Group, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University. All right reserved.
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